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KMID : 0364820110470030225
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Korean Journal of Microbiology
2011 Volume.47 No. 3 p.225 ~ p.230
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Production and Properties of Hemicellulases by an Isolate of Microbacterium sp.
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Yoon Ki-Hong
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Abstract
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A bacterium producing the extracellular mannanase and xylanase was isolated from Korean farm soil by successive subcultures in a minimal medium supplemented with palm kernel meal (PKM) and rice bran. The isolate YB-1106 showed 98% similarity with Microbacterium arabinogalactanolyticum on the basis of 16S rRNA gene sequences. The additional carbohydrates including locust bean gum (LBG) and PKM increased the mannanase productivity of the YB-1106, while the xylanase productivity of the isolate was increased by wheat bran, oat spelt xylan, rice bran and xy lose. Particularly, maximum mannanase and xylanase activities were obtained in the culture filtrate of tryptic soy broth supplemented with 1% LBG or 2% wheat bran, respectively. Both enzyme activities were produced at stationary growth phase. The mannanase of culture supernatant was the most active at 50°C and pH 6.0, while xylanase of culture supernatant was the most active at 55°C and pH 6.5. The predominant products resulting from the mannanase or xylanase hydrolysis were oligosaccharides for LBG or xylan, respectively.
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KEYWORD
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Microbacterium sp, mannanase, productivity, property, xylanase
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